Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)3
Start:864649 End:864660
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGTGCATTTATTTGCGCATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTAGCTCTGGGCCTCAAGT | |||||||||||||
| Tm_F_1 | 60.103 | Size_F_1 | 20 | Tm_R_1 | 60.012 | Size_R_1 | 20 | PRODUCT Size | 227 bp | Primer Start | 864602 | Primer End | 864828 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGTGCATTTATTTGCGCATT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTCAAAACCCACATCACCA | |||||||||||||
| Tm_F_2 | 60.103 | Size_F_2 | 20 | Tm_R_2 | 59.935 | Size_R_2 | 20 | PRODUCT Size | 104 bp | Primer Start | 864602 | Primer End | 864705 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTGCATTTATTTGCGCATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGGGACCCTTCCTCAGACT | |||||||||||||
| Tm_F_3 | 60.103 | Size_F_3 | 20 | Tm_R_3 | 59.929 | Size_R_3 | 20 | PRODUCT Size | 199 bp | Primer Start | 864602 | Primer End | 864800 |