Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)3
Start:561221 End:561232
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCAATGGAACCCTTTAGCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGGACGCTGAATCCAAAAG | |||||||||||||
| Tm_F_1 | 60.187 | Size_F_1 | 20 | Tm_R_1 | 60.074 | Size_R_1 | 20 | PRODUCT Size | 121 bp | Primer Start | 561165 | Primer End | 561285 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTTCCGTTGAAAGGATGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGGACGCTGAATCCAAAAG | |||||||||||||
| Tm_F_2 | 60.192 | Size_F_2 | 20 | Tm_R_2 | 60.074 | Size_R_2 | 20 | PRODUCT Size | 175 bp | Primer Start | 561111 | Primer End | 561285 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCAATGGAACCCTTTAGCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCTTCTCCTTTCCTCACGGA | |||||||||||||
| Tm_F_3 | 60.187 | Size_F_3 | 20 | Tm_R_3 | 59.92 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 561165 | Primer End | 561442 |