Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)3
Start:464119 End:464130
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAGAAGTTCCATCAGGGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTGGGCTGGATGAACTATC | |||||||||||||
| Tm_F_1 | 60.042 | Size_F_1 | 20 | Tm_R_1 | 59.658 | Size_R_1 | 20 | PRODUCT Size | 202 bp | Primer Start | 463980 | Primer End | 464181 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGTTCCATCAGGGGAGAGGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTGGGCTGGATGAACTATC | |||||||||||||
| Tm_F_2 | 59.929 | Size_F_2 | 20 | Tm_R_2 | 59.658 | Size_R_2 | 20 | PRODUCT Size | 196 bp | Primer Start | 463986 | Primer End | 464181 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAAGAAGTTCCATCAGGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATTGAAGGCGGGAAAGACT | |||||||||||||
| Tm_F_3 | 60.042 | Size_F_3 | 20 | Tm_R_3 | 60.578 | Size_R_3 | 20 | PRODUCT Size | 252 bp | Primer Start | 463980 | Primer End | 464231 |