Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGAA)3
Start:389139 End:389150
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCCTTCCCTCCTCCCTAAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGAAGGAGCTTTGCAGCTTT | |||||||||||||
| Tm_F_1 | 60.139 | Size_F_1 | 20 | Tm_R_1 | 60.27 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 388992 | Primer End | 389271 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCTTTCACTGACGTGGGTTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGGTGAAGGAGCTTTGCAG | |||||||||||||
| Tm_F_2 | 59.72 | Size_F_2 | 20 | Tm_R_2 | 60.134 | Size_R_2 | 20 | PRODUCT Size | 205 bp | Primer Start | 389071 | Primer End | 389275 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCTTTCACTGACGTGGGTTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGAGAGGTGAAGGAGCTTT | |||||||||||||
| Tm_F_3 | 59.72 | Size_F_3 | 20 | Tm_R_3 | 59.817 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 389071 | Primer End | 389279 |