Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGA)5
Start:114127 End:114141
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTCCAAAGAAAGCAGCACCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAGCTCCCTCTGGAACCTTT | |||||||||||||
| Tm_F_1 | 59.993 | Size_F_1 | 20 | Tm_R_1 | 59.817 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 113989 | Primer End | 114265 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGTCTCACAGCTTCTCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAGCTCCCTCTGGAACCTTT | |||||||||||||
| Tm_F_2 | 59.986 | Size_F_2 | 20 | Tm_R_2 | 59.817 | Size_R_2 | 20 | PRODUCT Size | 200 bp | Primer Start | 114066 | Primer End | 114265 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCTTTCAGTCCCCTCTCCTT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAGCTCCCTCTGGAACCTTT | |||||||||||||
| Tm_F_3 | 59.817 | Size_F_3 | 20 | Tm_R_3 | 59.817 | Size_R_3 | 20 | PRODUCT Size | 222 bp | Primer Start | 114044 | Primer End | 114265 |