Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGA)4aagatgaattcgacttaactccttatacctttttgacagt(GAAA)3
Start:731573 End:731636
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGGTCGAGCTCTTTCACCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GTTCAAGCAATGGAATTGGG | |||||||||||||
| Tm_F_1 | 59.989 | Size_F_1 | 20 | Tm_R_1 | 60.309 | Size_R_1 | 20 | PRODUCT Size | 277 bp | Primer Start | 731447 | Primer End | 731723 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGTCTCAGGGCTTCTAACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTCAAGCAATGGAATTGGG | |||||||||||||
| Tm_F_2 | 60.074 | Size_F_2 | 20 | Tm_R_2 | 60.309 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 731478 | Primer End | 731723 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAGGGCTTCTAACCTCTCCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTTCAAGCAATGGAATTGGG | |||||||||||||
| Tm_F_3 | 60.204 | Size_F_3 | 20 | Tm_R_3 | 60.309 | Size_R_3 | 20 | PRODUCT Size | 240 bp | Primer Start | 731484 | Primer End | 731723 |