Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGA)4
Start:920447 End:920458
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCTAGGCTGATCTCCTTCACC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCAAGGAAGGGACAAGTGA | |||||||||||||
| Tm_F_1 | 60.219 | Size_F_1 | 21 | Tm_R_1 | 59.549 | Size_R_1 | 20 | PRODUCT Size | 238 bp | Primer Start | 920284 | Primer End | 920521 |
| FORWARD_PRIMER_2 | |||||||||||||
| TGAACCTAGGCTGATCTCCTTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCAAGGAAGGGACAAGTGAA | |||||||||||||
| Tm_F_2 | 59.848 | Size_F_2 | 22 | Tm_R_2 | 60.081 | Size_R_2 | 20 | PRODUCT Size | 241 bp | Primer Start | 920280 | Primer End | 920520 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGGGAGTCAATCAAGACAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGACAAGTGAAGGAGTGAAGG | |||||||||||||
| Tm_F_3 | 59.903 | Size_F_3 | 20 | Tm_R_3 | 58.78 | Size_R_3 | 21 | PRODUCT Size | 280 bp | Primer Start | 920232 | Primer End | 920511 |