Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGA)4
Start:467535 End:467546
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TCGTCCAGTCATTAGCTGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCTGGCAAGGAATATCTGA | |||||||||||||
| Tm_F_1 | 60.03 | Size_F_1 | 21 | Tm_R_1 | 60.177 | Size_R_1 | 20 | PRODUCT Size | 175 bp | Primer Start | 467452 | Primer End | 467626 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCGGAACTGATATTCCCTG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTCGGAGAAGGTTCAGTCTCA | |||||||||||||
| Tm_F_2 | 59.887 | Size_F_2 | 20 | Tm_R_2 | 59.583 | Size_R_2 | 21 | PRODUCT Size | 256 bp | Primer Start | 467323 | Primer End | 467578 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTCGTCCAGTCATTAGCTGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCCTGGCAAGGAATATCTGA | |||||||||||||
| Tm_F_3 | 58.625 | Size_F_3 | 20 | Tm_R_3 | 60.177 | Size_R_3 | 20 | PRODUCT Size | 176 bp | Primer Start | 467451 | Primer End | 467626 |