Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AGA)4
Start:466577 End:466588
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGCTGGATGAGAGAGACGGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGAAAGGTTCTTCTTGACGC | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 59.993 | Size_R_1 | 20 | PRODUCT Size | 103 bp | Primer Start | 466539 | Primer End | 466641 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCACCTATTGATTCCTCGGG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTTTGTTTTCGCTTTCGTC | |||||||||||||
| Tm_F_2 | 59.887 | Size_F_2 | 20 | Tm_R_2 | 60.003 | Size_R_2 | 20 | PRODUCT Size | 251 bp | Primer Start | 466507 | Primer End | 466757 |
| FORWARD_PRIMER_3 | |||||||||||||
| CTGTTCCGTTCCGTCTCATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGAAAGGTTCTTCTTGACGC | |||||||||||||
| Tm_F_3 | 60.111 | Size_F_3 | 20 | Tm_R_3 | 59.993 | Size_R_3 | 20 | PRODUCT Size | 220 bp | Primer Start | 466422 | Primer End | 466641 |