Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ACTG)3atagtgagaaaaccaaatccggctaaataccaatacctatgac(AGGT)3
Start:752405 End:752471
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GACATCGGGAACCCCTAGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTCGATGAAATGGGCAGAAT | |||||||||||||
| Tm_F_1 | 60.154 | Size_F_1 | 20 | Tm_R_1 | 60.036 | Size_R_1 | 20 | PRODUCT Size | 269 bp | Primer Start | 752363 | Primer End | 752631 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACATCGGGAACCCCTAGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCAGAATGGCTATTCCAAA | |||||||||||||
| Tm_F_2 | 60.154 | Size_F_2 | 20 | Tm_R_2 | 60.038 | Size_R_2 | 20 | PRODUCT Size | 257 bp | Primer Start | 752363 | Primer End | 752619 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTAAGACATCGGGAACCCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTCGATGAAATGGGCAGAAT | |||||||||||||
| Tm_F_3 | 60.19 | Size_F_3 | 20 | Tm_R_3 | 60.036 | Size_R_3 | 20 | PRODUCT Size | 273 bp | Primer Start | 752359 | Primer End | 752631 |