Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ACCA)3
Start:472973 End:472984
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAAGCAAGACAAACTTGCCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGCCACTGCCTCACACTTA | |||||||||||||
| Tm_F_1 | 60.027 | Size_F_1 | 20 | Tm_R_1 | 60.049 | Size_R_1 | 20 | PRODUCT Size | 144 bp | Primer Start | 472908 | Primer End | 473051 |
| FORWARD_PRIMER_2 | |||||||||||||
| GACCCAGAAGAAACTCACGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTGCCACTGCCTCACACTTA | |||||||||||||
| Tm_F_2 | 59.851 | Size_F_2 | 20 | Tm_R_2 | 60.049 | Size_R_2 | 20 | PRODUCT Size | 181 bp | Primer Start | 472871 | Primer End | 473051 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAAGCAAGACAAACTTGCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CGGTATGGTACTCGTGACCC | |||||||||||||
| Tm_F_3 | 60.027 | Size_F_3 | 20 | Tm_R_3 | 60.255 | Size_R_3 | 20 | PRODUCT Size | 173 bp | Primer Start | 472908 | Primer End | 473080 |