Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ACCA)3
Start:240924 End:240935
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGCTTTCGAACTGACTCGAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGTGTAGGACTACCTCGGG | |||||||||||||
| Tm_F_1 | 59.997 | Size_F_1 | 20 | Tm_R_1 | 59.982 | Size_R_1 | 20 | PRODUCT Size | 261 bp | Primer Start | 240870 | Primer End | 241130 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTCAACCTTCGTTTCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATTCCATTGATAAGGCGTGC | |||||||||||||
| Tm_F_2 | 60 | Size_F_2 | 20 | Tm_R_2 | 59.929 | Size_R_2 | 20 | PRODUCT Size | 266 bp | Primer Start | 240767 | Primer End | 241032 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGCTTTCGAACTGACTCGAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATTCCATTGATAAGGCGTGC | |||||||||||||
| Tm_F_3 | 59.997 | Size_F_3 | 20 | Tm_R_3 | 59.929 | Size_R_3 | 20 | PRODUCT Size | 163 bp | Primer Start | 240870 | Primer End | 241032 |