Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(ACAA)3acctatcaagcattttttcggcaaatcatctctaggctttcaaaggcatccaaccaattggtatcccattgaaagat(AGAA)3
Start:267474 End:267574
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTGGATCAAGGCAGTGGATT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTGCTTGAAGCTAGTAGGCG | |||||||||||||
| Tm_F_1 | 59.934 | Size_F_1 | 20 | Tm_R_1 | 60.714 | Size_R_1 | 21 | PRODUCT Size | 233 bp | Primer Start | 267377 | Primer End | 267609 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGATCAAGGCAGTGGATTGT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTGCTTGAAGCTAGTAGGCG | |||||||||||||
| Tm_F_2 | 59.934 | Size_F_2 | 20 | Tm_R_2 | 60.714 | Size_R_2 | 21 | PRODUCT Size | 231 bp | Primer Start | 267379 | Primer End | 267609 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTGGATCAAGGCAGTGGATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CAGCCATTACATTTGTGCTTG | |||||||||||||
| Tm_F_3 | 59.934 | Size_F_3 | 20 | Tm_R_3 | 59.24 | Size_R_3 | 21 | PRODUCT Size | 246 bp | Primer Start | 267377 | Primer End | 267622 |