Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AATT)3
Start:247001 End:247012
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTATACTGCTTTTGGGCA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CCCACCTAACCTGGTATGGA | |||||||||||||
| Tm_F_1 | 59.467 | Size_F_1 | 20 | Tm_R_1 | 59.665 | Size_R_1 | 20 | PRODUCT Size | 263 bp | Primer Start | 246909 | Primer End | 247171 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAACCCTAGCCTAGTTGCCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CCTTTTTCGCGCCTAAAGTT | |||||||||||||
| Tm_F_2 | 60.262 | Size_F_2 | 20 | Tm_R_2 | 60.711 | Size_R_2 | 20 | PRODUCT Size | 210 bp | Primer Start | 246845 | Primer End | 247054 |
| FORWARD_PRIMER_3 | |||||||||||||
| CAACCCTAGCCTAGTTGCCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CTTTTTCGCGCCTAAAGTTC | |||||||||||||
| Tm_F_3 | 60.262 | Size_F_3 | 20 | Tm_R_3 | 59.143 | Size_R_3 | 20 | PRODUCT Size | 209 bp | Primer Start | 246845 | Primer End | 247053 |