Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AATC)3
Start:377718 End:377729
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACATACGGAAAGGTCAGGCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGCTTGTTGAGTCTCCCTTC | |||||||||||||
| Tm_F_1 | 58.672 | Size_F_1 | 20 | Tm_R_1 | 59.989 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 377657 | Primer End | 377924 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCTGACGTCTCGAGAAAGAGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGCCAGAGCGCAGGTAAGTA | |||||||||||||
| Tm_F_2 | 59.73 | Size_F_2 | 21 | Tm_R_2 | 60.176 | Size_R_2 | 20 | PRODUCT Size | 210 bp | Primer Start | 377558 | Primer End | 377767 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACATACGGAAAGGTCAGGCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGCCAGAGCGCAGGTAAGTA | |||||||||||||
| Tm_F_3 | 58.672 | Size_F_3 | 20 | Tm_R_3 | 60.176 | Size_R_3 | 20 | PRODUCT Size | 111 bp | Primer Start | 377657 | Primer End | 377767 |