Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGG)3
Start:520277 End:520288
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGAGCAAGTATGCGCTTTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCATCCCATTTCAACCAAA | |||||||||||||
| Tm_F_1 | 59.988 | Size_F_1 | 20 | Tm_R_1 | 60.029 | Size_R_1 | 20 | PRODUCT Size | 182 bp | Primer Start | 520152 | Primer End | 520333 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTACCGGTGGGTGGTTTTTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCATCCCATTTCAACCAAA | |||||||||||||
| Tm_F_2 | 60.081 | Size_F_2 | 20 | Tm_R_2 | 60.029 | Size_R_2 | 20 | PRODUCT Size | 115 bp | Primer Start | 520219 | Primer End | 520333 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTCGTCTTGGGGCTAAGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCATCCCATTTCAACCAAA | |||||||||||||
| Tm_F_3 | 60.132 | Size_F_3 | 20 | Tm_R_3 | 60.029 | Size_R_3 | 20 | PRODUCT Size | 275 bp | Primer Start | 520059 | Primer End | 520333 |