Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)4agaatcagtttcgaataggaacccaaaggcaggcgtgagtgcagtagacgtttgaaagactatatatatagaactcaaatctttcatccctgtttcga(AATAG)3
Start:685331 End:685459
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAGAGGAAAGAAAGGGGCAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCTGGGTAAAGAACTGCAA | |||||||||||||
| Tm_F_1 | 59.821 | Size_F_1 | 20 | Tm_R_1 | 60.249 | Size_R_1 | 20 | PRODUCT Size | 245 bp | Primer Start | 685255 | Primer End | 685499 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAGAGGAAAGAAAGGGGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGCTGGGTAAAGAACTGCAA | |||||||||||||
| Tm_F_2 | 60.18 | Size_F_2 | 20 | Tm_R_2 | 60.249 | Size_R_2 | 20 | PRODUCT Size | 246 bp | Primer Start | 685254 | Primer End | 685499 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAGCAGTAGGAGCCCTAAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGCTGGGTAAAGAACTGCAA | |||||||||||||
| Tm_F_3 | 59.615 | Size_F_3 | 20 | Tm_R_3 | 60.249 | Size_R_3 | 20 | PRODUCT Size | 263 bp | Primer Start | 685237 | Primer End | 685499 |