Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)4
Start:642523 End:642538
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGGTCCGGTCTCTTCTCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TACAGTTCAATCCGGGAAGG | |||||||||||||
| Tm_F_1 | 60.073 | Size_F_1 | 20 | Tm_R_1 | 59.926 | Size_R_1 | 20 | PRODUCT Size | 216 bp | Primer Start | 642487 | Primer End | 642702 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCGGTCTCTTCTCCTTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TACAGTTCAATCCGGGAAGG | |||||||||||||
| Tm_F_2 | 59.92 | Size_F_2 | 20 | Tm_R_2 | 59.926 | Size_R_2 | 20 | PRODUCT Size | 212 bp | Primer Start | 642491 | Primer End | 642702 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTGTGAGCTCCTTCCTCCT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACAATAACCGCACAGCACAA | |||||||||||||
| Tm_F_3 | 59.986 | Size_F_3 | 20 | Tm_R_3 | 60.18 | Size_R_3 | 20 | PRODUCT Size | 269 bp | Primer Start | 642340 | Primer End | 642608 |