Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)3
Start:564521 End:564532
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGAAAATTCCCAGAACAAGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTTCAACCGCGAGACCTTA | |||||||||||||
| Tm_F_1 | 58.823 | Size_F_1 | 21 | Tm_R_1 | 59.875 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 564492 | Primer End | 564757 |
| FORWARD_PRIMER_2 | |||||||||||||
| CAGAAAATTCCCAGAACAAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTCAACCGCGAGACCTTAC | |||||||||||||
| Tm_F_2 | 58.823 | Size_F_2 | 21 | Tm_R_2 | 59.74 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 564492 | Primer End | 564756 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACAGAAAATTCCCAGAACAAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTTCAACCGCGAGACCTTA | |||||||||||||
| Tm_F_3 | 59.637 | Size_F_3 | 22 | Tm_R_3 | 59.875 | Size_R_3 | 20 | PRODUCT Size | 267 bp | Primer Start | 564491 | Primer End | 564757 |