Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)3
Start:539545 End:539556
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTCGGTAAAGCAGCGTCTTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGCAAGAGGAGCAGATCAAG | |||||||||||||
| Tm_F_1 | 60.154 | Size_F_1 | 20 | Tm_R_1 | 60.096 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 539502 | Primer End | 539781 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCTCTGAGCCTGATTTCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAATCCGAGCGAAGACACTT | |||||||||||||
| Tm_F_2 | 59.957 | Size_F_2 | 20 | Tm_R_2 | 59.434 | Size_R_2 | 20 | PRODUCT Size | 174 bp | Primer Start | 539432 | Primer End | 539605 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTGACCCTTTGCCTTTACA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAATCCGAGCGAAGACACTT | |||||||||||||
| Tm_F_3 | 60.103 | Size_F_3 | 20 | Tm_R_3 | 59.434 | Size_R_3 | 20 | PRODUCT Size | 216 bp | Primer Start | 539390 | Primer End | 539605 |