Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)3
Start:538465 End:538476
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATGGTCCGGTCTCTTCTCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATCCTGCTTTCGGAAGGAAT | |||||||||||||
| Tm_F_1 | 60.073 | Size_F_1 | 20 | Tm_R_1 | 60.039 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 538420 | Primer End | 538656 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCGGTCTCTTCTCCTTTCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATCCTGCTTTCGGAAGGAAT | |||||||||||||
| Tm_F_2 | 59.92 | Size_F_2 | 20 | Tm_R_2 | 60.039 | Size_R_2 | 20 | PRODUCT Size | 233 bp | Primer Start | 538424 | Primer End | 538656 |
| FORWARD_PRIMER_3 | |||||||||||||
| CACAGTAAAGAAGGGCGAGC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAGCTCATATCGCGTGAG | |||||||||||||
| Tm_F_3 | 60.015 | Size_F_3 | 20 | Tm_R_3 | 60.119 | Size_R_3 | 20 | PRODUCT Size | 236 bp | Primer Start | 538376 | Primer End | 538611 |