Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAGA)3
Start:173989 End:174000
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCTGAAGAGTGGATCCGTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TAAGAGCCAAATTGTCCCCA | |||||||||||||
| Tm_F_1 | 60.218 | Size_F_1 | 20 | Tm_R_1 | 60.439 | Size_R_1 | 20 | PRODUCT Size | 262 bp | Primer Start | 173769 | Primer End | 174030 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCCTGAAGAGTGGATCCGTA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGCGCAGTTAAGAGCCAAAT | |||||||||||||
| Tm_F_2 | 60.218 | Size_F_2 | 20 | Tm_R_2 | 60.905 | Size_R_2 | 20 | PRODUCT Size | 270 bp | Primer Start | 173769 | Primer End | 174038 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGAAGGCCGCAATACATCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TAAGAGCCAAATTGTCCCCA | |||||||||||||
| Tm_F_3 | 59.297 | Size_F_3 | 20 | Tm_R_3 | 60.439 | Size_R_3 | 20 | PRODUCT Size | 201 bp | Primer Start | 173830 | Primer End | 174030 |