Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)6
Start:827250 End:827267
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAACATAGGCCAGCCAGGAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCTGCGCTCCTCTTTATT | |||||||||||||
| Tm_F_1 | 60.227 | Size_F_1 | 20 | Tm_R_1 | 60.117 | Size_R_1 | 20 | PRODUCT Size | 151 bp | Primer Start | 827215 | Primer End | 827365 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAACATAGGCCAGCCAGGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GTTGCTGCGCTCCTCTTTAT | |||||||||||||
| Tm_F_2 | 60.227 | Size_F_2 | 20 | Tm_R_2 | 59.62 | Size_R_2 | 20 | PRODUCT Size | 152 bp | Primer Start | 827215 | Primer End | 827366 |
| FORWARD_PRIMER_3 | |||||||||||||
| TAACATAGGCCAGCCAGGAG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCCCTATTTTTCAGGGCA | |||||||||||||
| Tm_F_3 | 60.227 | Size_F_3 | 20 | Tm_R_3 | 60.439 | Size_R_3 | 20 | PRODUCT Size | 258 bp | Primer Start | 827215 | Primer End | 827472 |