Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)5
Start:969081 End:969095
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGAGAAGCGACTGAACCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGATAGGAAAAGGGTTTCGG | |||||||||||||
| Tm_F_1 | 59.615 | Size_F_1 | 20 | Tm_R_1 | 59.772 | Size_R_1 | 20 | PRODUCT Size | 235 bp | Primer Start | 968992 | Primer End | 969226 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCGACTGAACCGATATGACC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GGAAAAGGGTTTCGGTTGAT | |||||||||||||
| Tm_F_2 | 60.492 | Size_F_2 | 20 | Tm_R_2 | 60.166 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 969000 | Primer End | 969221 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCGACTGAACCGATATGACC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGATAGGAAAAGGGTTTCGG | |||||||||||||
| Tm_F_3 | 60.492 | Size_F_3 | 20 | Tm_R_3 | 59.772 | Size_R_3 | 20 | PRODUCT Size | 227 bp | Primer Start | 969000 | Primer End | 969226 |