Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)5
Start:889908 End:889922
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AAAGAATCCAGAGGTTCCCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CTGGGTAAAGCGTCTTCCCT | |||||||||||||
| Tm_F_1 | 59.373 | Size_F_1 | 20 | Tm_R_1 | 60.621 | Size_R_1 | 20 | PRODUCT Size | 268 bp | Primer Start | 889836 | Primer End | 890103 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCAGAATCAAAGGGAAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GATTCAATGAGGGTTGGTTGA | |||||||||||||
| Tm_F_2 | 59.955 | Size_F_2 | 20 | Tm_R_2 | 59.782 | Size_R_2 | 21 | PRODUCT Size | 280 bp | Primer Start | 889726 | Primer End | 890005 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACACCGGTCAGGGAATATCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GATTCAATGAGGGTTGGTTGA | |||||||||||||
| Tm_F_3 | 60.195 | Size_F_3 | 20 | Tm_R_3 | 59.782 | Size_R_3 | 21 | PRODUCT Size | 204 bp | Primer Start | 889802 | Primer End | 890005 |