Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)5
Start:514460 End:514474
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACTCTTTCGTAAAGCGCTGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTTCCGGTTCTTCCTTCA | |||||||||||||
| Tm_F_1 | 59.797 | Size_F_1 | 20 | Tm_R_1 | 60.605 | Size_R_1 | 20 | PRODUCT Size | 178 bp | Primer Start | 514338 | Primer End | 514515 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACTCTTTCGTAAAGCGCTGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTCCGGTTCTTCCTTCAA | |||||||||||||
| Tm_F_2 | 59.797 | Size_F_2 | 20 | Tm_R_2 | 60.605 | Size_R_2 | 20 | PRODUCT Size | 177 bp | Primer Start | 514338 | Primer End | 514514 |
| FORWARD_PRIMER_3 | |||||||||||||
| AGAACCTGCCCCTCTTCCTA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTTCCGGTTCTTCCTTCA | |||||||||||||
| Tm_F_3 | 60.204 | Size_F_3 | 20 | Tm_R_3 | 60.605 | Size_R_3 | 20 | PRODUCT Size | 151 bp | Primer Start | 514365 | Primer End | 514515 |