Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)4gatgaatgagacagattgaactcttgaacaaggtaaagc(GGCT)3
Start:493530 End:493592
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGGAACCAGATTGAACTGT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GAAGCAGCTTTACCGACAGG | |||||||||||||
| Tm_F_1 | 59.966 | Size_F_1 | 20 | Tm_R_1 | 60.015 | Size_R_1 | 20 | PRODUCT Size | 248 bp | Primer Start | 493502 | Primer End | 493749 |
| FORWARD_PRIMER_2 | |||||||||||||
| TACTCCTGAACCGGAACCAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAAGCAGCTTTACCGACAGG | |||||||||||||
| Tm_F_2 | 60.103 | Size_F_2 | 20 | Tm_R_2 | 60.015 | Size_R_2 | 20 | PRODUCT Size | 258 bp | Primer Start | 493492 | Primer End | 493749 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCGGAACCAGATTGAACTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGGAATATCGTCTTTGGGG | |||||||||||||
| Tm_F_3 | 59.966 | Size_F_3 | 20 | Tm_R_3 | 60.145 | Size_R_3 | 20 | PRODUCT Size | 150 bp | Primer Start | 493502 | Primer End | 493651 |