Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)4
Start:917791 End:917802
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AGGAAAAGGGGAAGGAGCTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGTTCCTGAACCTTCACCC | |||||||||||||
| Tm_F_1 | 60.547 | Size_F_1 | 20 | Tm_R_1 | 59.943 | Size_R_1 | 20 | PRODUCT Size | 247 bp | Primer Start | 917593 | Primer End | 917839 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAAAGGGGAAGGAGCTTGAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTCACCCACGCATCCTTTAT | |||||||||||||
| Tm_F_2 | 59.691 | Size_F_2 | 20 | Tm_R_2 | 60.331 | Size_R_2 | 20 | PRODUCT Size | 231 bp | Primer Start | 917596 | Primer End | 917826 |
| FORWARD_PRIMER_3 | |||||||||||||
| GGGAAGGAGCTTGACCTTGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTTCCTGAACCTTCACCC | |||||||||||||
| Tm_F_3 | 60.628 | Size_F_3 | 20 | Tm_R_3 | 59.943 | Size_R_3 | 20 | PRODUCT Size | 239 bp | Primer Start | 917601 | Primer End | 917839 |