Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)4
Start:755149 End:755160
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GAACCAGTCCCAACGAGAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGGAATACCTACCGTAGCA | |||||||||||||
| Tm_F_1 | 60.088 | Size_F_1 | 20 | Tm_R_1 | 59.975 | Size_R_1 | 20 | PRODUCT Size | 280 bp | Primer Start | 755034 | Primer End | 755313 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACCAGTCCCAACGAGAAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTCGGTCATACGACACGTT | |||||||||||||
| Tm_F_2 | 60.088 | Size_F_2 | 20 | Tm_R_2 | 60.032 | Size_R_2 | 20 | PRODUCT Size | 190 bp | Primer Start | 755034 | Primer End | 755223 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACTGGGCGTTTGCTAAAAGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGTCGGTCATACGACACGTT | |||||||||||||
| Tm_F_3 | 59.883 | Size_F_3 | 20 | Tm_R_3 | 60.032 | Size_R_3 | 20 | PRODUCT Size | 239 bp | Primer Start | 754985 | Primer End | 755223 |