Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)4
Start:542418 End:542429
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTGACCGTAAGGAGGTAAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGGTTGAGGTTCAGTCTTTGA | |||||||||||||
| Tm_F_1 | 59.755 | Size_F_1 | 21 | Tm_R_1 | 59.173 | Size_R_1 | 21 | PRODUCT Size | 263 bp | Primer Start | 542211 | Primer End | 542473 |
| FORWARD_PRIMER_2 | |||||||||||||
| AAGAGAGAGAGAATGGGCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CAAGGGTGAAGTAACTCAGGG | |||||||||||||
| Tm_F_2 | 60.862 | Size_F_2 | 20 | Tm_R_2 | 58.705 | Size_R_2 | 21 | PRODUCT Size | 110 bp | Primer Start | 542382 | Primer End | 542491 |
| FORWARD_PRIMER_3 | |||||||||||||
| AAGAGAGAGAGAATGGGCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGGTGAAGTAACTCAGGGTTGA | |||||||||||||
| Tm_F_3 | 60.862 | Size_F_3 | 20 | Tm_R_3 | 60.395 | Size_R_3 | 22 | PRODUCT Size | 107 bp | Primer Start | 542382 | Primer End | 542488 |