Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAG)4
Start:407556 End:407567
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCTGCGCTCCTCTTTCTTTA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| AGTCTTTCCTCTCCCTTCCG | |||||||||||||
| Tm_F_1 | 59.867 | Size_F_1 | 20 | Tm_R_1 | 59.811 | Size_R_1 | 20 | PRODUCT Size | 263 bp | Primer Start | 407429 | Primer End | 407691 |
| FORWARD_PRIMER_2 | |||||||||||||
| TAGGGGGAGGGATGATTAGC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGTCTTTCCTCTCCCTTCCG | |||||||||||||
| Tm_F_2 | 60.245 | Size_F_2 | 20 | Tm_R_2 | 59.811 | Size_R_2 | 20 | PRODUCT Size | 226 bp | Primer Start | 407466 | Primer End | 407691 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGGTAAAGCTTACGGTCTCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GTCTCGCACTTCGTTCCTCT | |||||||||||||
| Tm_F_3 | 59.898 | Size_F_3 | 20 | Tm_R_3 | 59.604 | Size_R_3 | 20 | PRODUCT Size | 237 bp | Primer Start | 407407 | Primer End | 407643 |