Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AACTA)3
Start:913676 End:913690
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACTCCCGAACTACCGTCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCGGTGTGCTCCTTACTC | |||||||||||||
| Tm_F_1 | 59.994 | Size_F_1 | 20 | Tm_R_1 | 60.02 | Size_R_1 | 20 | PRODUCT Size | 273 bp | Primer Start | 913519 | Primer End | 913791 |
| FORWARD_PRIMER_2 | |||||||||||||
| ATTCCCACCCTTTGACTCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTCGGTGTGCTCCTTACTC | |||||||||||||
| Tm_F_2 | 59.795 | Size_F_2 | 20 | Tm_R_2 | 60.02 | Size_R_2 | 20 | PRODUCT Size | 156 bp | Primer Start | 913636 | Primer End | 913791 |
| FORWARD_PRIMER_3 | |||||||||||||
| GACTCCTCCCTGCCTACTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCTCGGTGTGCTCCTTACTC | |||||||||||||
| Tm_F_3 | 60.216 | Size_F_3 | 20 | Tm_R_3 | 60.02 | Size_R_3 | 20 | PRODUCT Size | 143 bp | Primer Start | 913649 | Primer End | 913791 |