Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AACT)5
Start:375649 End:375668
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTTGAACCACTCTTTGACTG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGACCTTTCATCCGGCTTAG | |||||||||||||
| Tm_F_1 | 60.465 | Size_F_1 | 22 | Tm_R_1 | 60.206 | Size_R_1 | 20 | PRODUCT Size | 278 bp | Primer Start | 375574 | Primer End | 375851 |
| FORWARD_PRIMER_2 | |||||||||||||
| GCTTGAACCACTCTTTGACTGA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGACCTTTCATCCGGCTTAG | |||||||||||||
| Tm_F_2 | 59.52 | Size_F_2 | 22 | Tm_R_2 | 60.206 | Size_R_2 | 20 | PRODUCT Size | 277 bp | Primer Start | 375575 | Primer End | 375851 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGTTGACTCTACGACTGAAGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGACCTTTCATCCGGCTTAG | |||||||||||||
| Tm_F_3 | 59.396 | Size_F_3 | 22 | Tm_R_3 | 60.206 | Size_R_3 | 20 | PRODUCT Size | 229 bp | Primer Start | 375623 | Primer End | 375851 |