Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AACT)3
Start:533842 End:533853
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CAGTTACTCGCCGACTGAAG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGTACGAGCTTGACCTTCC | |||||||||||||
| Tm_F_1 | 58.675 | Size_F_1 | 20 | Tm_R_1 | 59.357 | Size_R_1 | 20 | PRODUCT Size | 252 bp | Primer Start | 533816 | Primer End | 534067 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCAGTTACTCGCCGACTGAAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGTACGAGCTTGACCTTCC | |||||||||||||
| Tm_F_2 | 60.577 | Size_F_2 | 21 | Tm_R_2 | 59.357 | Size_R_2 | 20 | PRODUCT Size | 253 bp | Primer Start | 533815 | Primer End | 534067 |
| FORWARD_PRIMER_3 | |||||||||||||
| AATTCAGTTACTCGCCGACTG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACGTACGAGCTTGACCTTCC | |||||||||||||
| Tm_F_3 | 59.392 | Size_F_3 | 21 | Tm_R_3 | 59.357 | Size_R_3 | 20 | PRODUCT Size | 256 bp | Primer Start | 533812 | Primer End | 534067 |