Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AACA)3
Start:4835 End:4846
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GGGGGATTCCTCTTTCACTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CGGAGAATGGAATGAATGGT | |||||||||||||
| Tm_F_1 | 59.874 | Size_F_1 | 20 | Tm_R_1 | 59.75 | Size_R_1 | 20 | PRODUCT Size | 222 bp | Primer Start | 4746 | Primer End | 4967 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGGGGATTCCTCTTTCACTC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCTTTCGTCTTGCCTTCCTG | |||||||||||||
| Tm_F_2 | 59.874 | Size_F_2 | 20 | Tm_R_2 | 60.508 | Size_R_2 | 20 | PRODUCT Size | 245 bp | Primer Start | 4746 | Primer End | 4990 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGGTAAACTGTTAGGGGGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGAAACCCCAGTGAAAGAGG | |||||||||||||
| Tm_F_3 | 59.284 | Size_F_3 | 20 | Tm_R_3 | 60.081 | Size_R_3 | 20 | PRODUCT Size | 192 bp | Primer Start | 4732 | Primer End | 4923 |