Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAC)6aagaacaatttacattacggaggaggaaggtaaagctactcccctaacg(TCTAAC)3ttctaactct(CTCTAA)3
Start:639664 End:639776
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCCCTGACGAAGACAACAAC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGTCTGGACGTCAGGTAAGC | |||||||||||||
| Tm_F_1 | 60.545 | Size_F_1 | 20 | Tm_R_1 | 58.882 | Size_R_1 | 20 | PRODUCT Size | 259 bp | Primer Start | 639589 | Primer End | 639847 |
| FORWARD_PRIMER_2 | |||||||||||||
| GGAAGGAAAAGCTACTCCCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGTTTGTCTGGACGTCAGGT | |||||||||||||
| Tm_F_2 | 59.171 | Size_F_2 | 20 | Tm_R_2 | 59.152 | Size_R_2 | 20 | PRODUCT Size | 222 bp | Primer Start | 639630 | Primer End | 639851 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCCCTGACGAAGACAACAAC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTGTCTGGACGTCAGGTAAGC | |||||||||||||
| Tm_F_3 | 60.545 | Size_F_3 | 20 | Tm_R_3 | 60.306 | Size_R_3 | 21 | PRODUCT Size | 260 bp | Primer Start | 639589 | Primer End | 639848 |