Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAGA)3
Start:261415 End:261429
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ATAGGGCTGGCTGACAGAGA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| CACTATTCCGGGACTGGGTA | |||||||||||||
| Tm_F_1 | 59.973 | Size_F_1 | 20 | Tm_R_1 | 59.807 | Size_R_1 | 20 | PRODUCT Size | 249 bp | Primer Start | 261223 | Primer End | 261471 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGCAAACATTCAAACGGGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CACTATTCCGGGACTGGGTA | |||||||||||||
| Tm_F_2 | 60.11 | Size_F_2 | 20 | Tm_R_2 | 59.807 | Size_R_2 | 20 | PRODUCT Size | 131 bp | Primer Start | 261341 | Primer End | 261471 |
| FORWARD_PRIMER_3 | |||||||||||||
| CCTACAGGCCTAAACGACGA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CACTATTCCGGGACTGGGTA | |||||||||||||
| Tm_F_3 | 60.262 | Size_F_3 | 20 | Tm_R_3 | 59.807 | Size_R_3 | 20 | PRODUCT Size | 150 bp | Primer Start | 261322 | Primer End | 261471 |