Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)5
Start:842542 End:842561
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACTATGACTCCCGCTCCCT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GGAATCCCTAAAAAGCCCAG | |||||||||||||
| Tm_F_1 | 60.096 | Size_F_1 | 20 | Tm_R_1 | 59.903 | Size_R_1 | 20 | PRODUCT Size | 243 bp | Primer Start | 842436 | Primer End | 842678 |
| FORWARD_PRIMER_2 | |||||||||||||
| AACTATGACTCCCGCTCCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GAATCCCTAAAAAGCCCAGG | |||||||||||||
| Tm_F_2 | 60.096 | Size_F_2 | 20 | Tm_R_2 | 59.903 | Size_R_2 | 20 | PRODUCT Size | 242 bp | Primer Start | 842436 | Primer End | 842677 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGGAACTATGACTCCCGCTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GAATCCCTAAAAAGCCCAGG | |||||||||||||
| Tm_F_3 | 60.218 | Size_F_3 | 20 | Tm_R_3 | 59.903 | Size_R_3 | 20 | PRODUCT Size | 245 bp | Primer Start | 842433 | Primer End | 842677 |