Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)4a(ATTG)3
Start:148382 End:148410
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCAGCTTCTCCATCAACTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TTGCTTTTCTTTCCTCTCGC | |||||||||||||
| Tm_F_1 | 59.957 | Size_F_1 | 20 | Tm_R_1 | 59.708 | Size_R_1 | 20 | PRODUCT Size | 190 bp | Primer Start | 148357 | Primer End | 148546 |
| FORWARD_PRIMER_2 | |||||||||||||
| TCCAACCCAGTTCCAACAAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TTGCTTTTCTTTCCTCTCGC | |||||||||||||
| Tm_F_2 | 60.21 | Size_F_2 | 20 | Tm_R_2 | 59.708 | Size_R_2 | 20 | PRODUCT Size | 214 bp | Primer Start | 148333 | Primer End | 148546 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCAGCTTCTCCATCAACTCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| CCTCTCAGTTCGTGTTCGTG | |||||||||||||
| Tm_F_3 | 59.957 | Size_F_3 | 20 | Tm_R_3 | 59.461 | Size_R_3 | 20 | PRODUCT Size | 245 bp | Primer Start | 148357 | Primer End | 148601 |