Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)4
Start:492631 End:492646
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TACCTAGCCTCTGTCCGCAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCTCCTGGCTTTCTTTTCCT | |||||||||||||
| Tm_F_1 | 59.859 | Size_F_1 | 20 | Tm_R_1 | 59.962 | Size_R_1 | 20 | PRODUCT Size | 158 bp | Primer Start | 492532 | Primer End | 492689 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCATGCTACCCTTCTTCGAG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCTCCTGGCTTTCTTTTCCT | |||||||||||||
| Tm_F_2 | 59.83 | Size_F_2 | 20 | Tm_R_2 | 59.962 | Size_R_2 | 20 | PRODUCT Size | 274 bp | Primer Start | 492416 | Primer End | 492689 |
| FORWARD_PRIMER_3 | |||||||||||||
| TACCTAGCCTCTGTCCGCAT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCCTGGCTTTCTTTTCCTCA | |||||||||||||
| Tm_F_3 | 59.859 | Size_F_3 | 20 | Tm_R_3 | 59.926 | Size_R_3 | 20 | PRODUCT Size | 156 bp | Primer Start | 492532 | Primer End | 492687 |