Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)3aaaatggatcaatcagagaatattcatcttgacaac(AAGA)3
Start:725223 End:725282
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GCCGGATGAAAGCAATACTC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCCCTTGCGTGATACTTCTC | |||||||||||||
| Tm_F_1 | 59.674 | Size_F_1 | 20 | Tm_R_1 | 59.843 | Size_R_1 | 20 | PRODUCT Size | 237 bp | Primer Start | 725086 | Primer End | 725322 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTTTGAACCAATGACTCCCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCCCTTGCGTGATACTTCTC | |||||||||||||
| Tm_F_2 | 60.353 | Size_F_2 | 20 | Tm_R_2 | 59.843 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 725060 | Primer End | 725322 |
| FORWARD_PRIMER_3 | |||||||||||||
| GCCGGATGAAAGCAATACTC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GGAAACGAGGTGCTCTACCA | |||||||||||||
| Tm_F_3 | 59.674 | Size_F_3 | 20 | Tm_R_3 | 60.255 | Size_R_3 | 20 | PRODUCT Size | 268 bp | Primer Start | 725086 | Primer End | 725353 |