Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)3
Start:8191 End:8202
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TATGGGGGATGGATAAGCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| GCGGGTAAACGACTCTACCA | |||||||||||||
| Tm_F_1 | 60.11 | Size_F_1 | 20 | Tm_R_1 | 60.132 | Size_R_1 | 20 | PRODUCT Size | 130 bp | Primer Start | 8167 | Primer End | 8296 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTATGGGGGATGGATAAGCA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| GCGGGTAAACGACTCTACCA | |||||||||||||
| Tm_F_2 | 60.11 | Size_F_2 | 20 | Tm_R_2 | 60.132 | Size_R_2 | 20 | PRODUCT Size | 131 bp | Primer Start | 8166 | Primer End | 8296 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATTTGAACCAATGACTCCCG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| GCGGGTAAACGACTCTACCA | |||||||||||||
| Tm_F_3 | 59.79 | Size_F_3 | 20 | Tm_R_3 | 60.132 | Size_R_3 | 20 | PRODUCT Size | 249 bp | Primer Start | 8048 | Primer End | 8296 |