Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)3
Start:540348 End:540359
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGCTGCCCTATGCTTTCTTT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCAGGTTCAGTAGCCAAGGG | |||||||||||||
| Tm_F_1 | 59.982 | Size_F_1 | 20 | Tm_R_1 | 60.246 | Size_R_1 | 20 | PRODUCT Size | 279 bp | Primer Start | 540109 | Primer End | 540387 |
| FORWARD_PRIMER_2 | |||||||||||||
| TTGCTGCCCTATGCTTTCTT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TCAGGTTCAGTAGCCAAGGG | |||||||||||||
| Tm_F_2 | 59.982 | Size_F_2 | 20 | Tm_R_2 | 60.246 | Size_R_2 | 20 | PRODUCT Size | 280 bp | Primer Start | 540108 | Primer End | 540387 |
| FORWARD_PRIMER_3 | |||||||||||||
| GTTTACGGCGGGAAGTAGGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGGTCGGGGAAGAGAAAGAA | |||||||||||||
| Tm_F_3 | 60.373 | Size_F_3 | 20 | Tm_R_3 | 60.183 | Size_R_3 | 20 | PRODUCT Size | 141 bp | Primer Start | 540307 | Primer End | 540447 |