Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAG)3
Start:417013 End:417024
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TTGTAATGGAACGAACGCAA | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACGCGGTCTATGAAACCAGA | |||||||||||||
| Tm_F_1 | 60.111 | Size_F_1 | 20 | Tm_R_1 | 60.657 | Size_R_1 | 20 | PRODUCT Size | 271 bp | Primer Start | 416822 | Primer End | 417092 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAACGAACGCAATAGCACAA | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACGCGGTCTATGAAACCAGA | |||||||||||||
| Tm_F_2 | 59.879 | Size_F_2 | 20 | Tm_R_2 | 60.657 | Size_R_2 | 20 | PRODUCT Size | 263 bp | Primer Start | 416830 | Primer End | 417092 |
| FORWARD_PRIMER_3 | |||||||||||||
| TTTTTCTTGCTAGGTTGGGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TTCTTTCCCTGTGGAATTGG | |||||||||||||
| Tm_F_3 | 59.198 | Size_F_3 | 20 | Tm_R_3 | 59.903 | Size_R_3 | 20 | PRODUCT Size | 278 bp | Primer Start | 416882 | Primer End | 417159 |