Primers Available
Organism:Cucurbita pepo mitochondrion
Repeat:(AAAC)3cccgatt(CAAA)3
Start:297841 End:297871
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TAGCCCGGTCAGTGTAATCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TGGAGAATCAATCCCCAGAG | |||||||||||||
| Tm_F_1 | 59.955 | Size_F_1 | 20 | Tm_R_1 | 60.003 | Size_R_1 | 20 | PRODUCT Size | 136 bp | Primer Start | 297811 | Primer End | 297946 |
| FORWARD_PRIMER_2 | |||||||||||||
| CACATCCCCATTAGGACCAC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| TGGAGAATCAATCCCCAGAG | |||||||||||||
| Tm_F_2 | 60.05 | Size_F_2 | 20 | Tm_R_2 | 60.003 | Size_R_2 | 20 | PRODUCT Size | 158 bp | Primer Start | 297789 | Primer End | 297946 |
| FORWARD_PRIMER_3 | |||||||||||||
| ATGTCGCTATTCCTGCATCC | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TGGAGAATCAATCCCCAGAG | |||||||||||||
| Tm_F_3 | 60.066 | Size_F_3 | 20 | Tm_R_3 | 60.003 | Size_R_3 | 20 | PRODUCT Size | 228 bp | Primer Start | 297719 | Primer End | 297946 |