Primers Available
Organism: Triploid Megalobrama amblycephala x Xenocypris davidi mitochondrion
Repeat:(TA)8
Start:814 End:829
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| AACCCCGAAACCAAGGAAGG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACTTTTTAGCATTGGGGCGC | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 59.754 | Size_R_1 | 20 | PRODUCT Size | 179 bp | Primer Start | 743 | Primer End | 921 |
| FORWARD_PRIMER_2 | |||||||||||||
| CCCTACGCTCAGCAAATCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACTTTTTAGCATTGGGGCGC | |||||||||||||
| Tm_F_2 | 59.821 | Size_F_2 | 20 | Tm_R_2 | 59.754 | Size_R_2 | 20 | PRODUCT Size | 212 bp | Primer Start | 710 | Primer End | 921 |
| FORWARD_PRIMER_3 | |||||||||||||
| AACCCCGAAACCAAGGAAGG | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| TCGGGACCATGCCTTTATGC | |||||||||||||
| Tm_F_3 | 60.179 | Size_F_3 | 20 | Tm_R_3 | 60.465 | Size_R_3 | 20 | PRODUCT Size | 280 bp | Primer Start | 743 | Primer End | 1022 |