Primers Available
Organism: Amphipholis squamata mitochondrion
Repeat:(AATA)3
Start:5249 End:5260
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| GTGGAAATCCGCCAAGTGAT | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| TCGAAAGATTTTAGCGCTTTCTTCA | |||||||||||||
| Tm_F_1 | 58.536 | Size_F_1 | 20 | Tm_R_1 | 59.821 | Size_R_1 | 25 | PRODUCT Size | 242 bp | Primer Start | 5114 | Primer End | 5355 |
| FORWARD_PRIMER_2 | |||||||||||||
| GTGGAAATCCGCCAAGTGAT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| AGGGTGGCTTTTATTAGGTTTACC | |||||||||||||
| Tm_F_2 | 58.536 | Size_F_2 | 20 | Tm_R_2 | 58.733 | Size_R_2 | 24 | PRODUCT Size | 206 bp | Primer Start | 5114 | Primer End | 5319 |
| FORWARD_PRIMER_3 | |||||||||||||
| ACCCATAATAAAGGAGCCACCA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| AGTTCAGTAGCTGTAGGAAGC | |||||||||||||
| Tm_F_3 | 59.144 | Size_F_3 | 22 | Tm_R_3 | 57.122 | Size_R_3 | 21 | PRODUCT Size | 179 bp | Primer Start | 5224 | Primer End | 5402 |