Primers Available
Organism: Amphipholis squamata mitochondrion
Repeat:(AAAG)3
Start:3669 End:3680
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CCGCTTACAAAACGAGTCGC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ATGCTTGCTGTTTTCGTCCT | |||||||||||||
| Tm_F_1 | 60.179 | Size_F_1 | 20 | Tm_R_1 | 58.391 | Size_R_1 | 20 | PRODUCT Size | 240 bp | Primer Start | 3576 | Primer End | 3815 |
| FORWARD_PRIMER_2 | |||||||||||||
| AGGCTTCCTCTGGTCTCTCC | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ATGCTTGCTGTTTTCGTCCT | |||||||||||||
| Tm_F_2 | 60.325 | Size_F_2 | 20 | Tm_R_2 | 58.391 | Size_R_2 | 20 | PRODUCT Size | 260 bp | Primer Start | 3556 | Primer End | 3815 |
| FORWARD_PRIMER_3 | |||||||||||||
| TCCCCGCTTACAAAACGAGT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ATGCTTGCTGTTTTCGTCCT | |||||||||||||
| Tm_F_3 | 59.606 | Size_F_3 | 20 | Tm_R_3 | 58.391 | Size_R_3 | 20 | PRODUCT Size | 243 bp | Primer Start | 3573 | Primer End | 3815 |