Primers Available
Organism: Ophiocomina nigra mitochondrion
Repeat:(ACC)4
Start:10925 End:10936
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ACCAATCGCAGGAGCTATCG | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCACGTGTGTAGTGTGCAT | |||||||||||||
| Tm_F_1 | 59.967 | Size_F_1 | 20 | Tm_R_1 | 59.894 | Size_R_1 | 20 | PRODUCT Size | 180 bp | Primer Start | 10896 | Primer End | 11075 |
| FORWARD_PRIMER_2 | |||||||||||||
| ACCAATCGCAGGAGCTATCG | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| CTTTATGCCTCGGACCACGT | |||||||||||||
| Tm_F_2 | 59.967 | Size_F_2 | 20 | Tm_R_2 | 60.108 | Size_R_2 | 20 | PRODUCT Size | 193 bp | Primer Start | 10896 | Primer End | 11088 |
| FORWARD_PRIMER_3 | |||||||||||||
| CGACCGAAGTCCATGACCAA | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCACGTGTGTAGTGTGCAT | |||||||||||||
| Tm_F_3 | 60.038 | Size_F_3 | 20 | Tm_R_3 | 59.894 | Size_R_3 | 20 | PRODUCT Size | 195 bp | Primer Start | 10881 | Primer End | 11075 |