Primers Available
Organism: Metastrongylus pudendotectus mitochondrion
Repeat:(TTA)4
Start:7676 End:7687
| FORWARD_PRIMER_1 | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| TGAAAATGAGAATTAAGGTTTTGTTCC | |||||||||||||
| REVERSE_PRIMER_1 | |||||||||||||
| ACCCAATAAATGACGCCCCC | |||||||||||||
| Tm_F_1 | 57.459 | Size_F_1 | 27 | Tm_R_1 | 60.397 | Size_R_1 | 20 | PRODUCT Size | 266 bp | Primer Start | 7521 | Primer End | 7786 |
| FORWARD_PRIMER_2 | |||||||||||||
| GAAAATGAGAATTAAGGTTTTGTTCCT | |||||||||||||
| REVERSE_PRIMER_2 | |||||||||||||
| ACCCAATAAATGACGCCCCC | |||||||||||||
| Tm_F_2 | 57.236 | Size_F_2 | 27 | Tm_R_2 | 60.397 | Size_R_2 | 20 | PRODUCT Size | 265 bp | Primer Start | 7522 | Primer End | 7786 |
| FORWARD_PRIMER_3 | |||||||||||||
| TGAGTTCTTGTTTTGAAAATGAGAATT | |||||||||||||
| REVERSE_PRIMER_3 | |||||||||||||
| ACCCAATAAATGACGCCCCC | |||||||||||||
| Tm_F_3 | 57.126 | Size_F_3 | 27 | Tm_R_3 | 60.397 | Size_R_3 | 20 | PRODUCT Size | 279 bp | Primer Start | 7508 | Primer End | 7786 |